Analysis Note

Positive ControlAll animal cell lines

Application

Immunoblotting (1:5000-1:20000)r>Immunofluorescence (1:10000, see application references)r>r>Recomended dilution for secondary antibody (0.05 µg/ml, 1:2000 dilution)

General description

Mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells supplied as ascites. Recognizes the ~42 kDa α-, β- and γ-isoforms of actin. Supplied with goat anti-mouse IgM conjugated to horseradish peroxidase.

Anti-Actin (Ab-1), mouse monoclonal, clone JLA20, recognizes the ~ 42 kDa α-, β-, and γ isoforms of actin. It is validated for Western blotting and immunofluorescence.

Recognizes the ~42 kDa α-, β-, and γ isoforms of actin. Useful as a positive control antibody.Antibody Target Gene Symbol: ACTA1, ACTB1, ACTG1 Target Synonym: AA959943, ACTA, Acta-2, Acts, Actsk-1, ALPHA SKELETAL ACTIN, ALPHA1 ACTIN, ASMA, CFTD, CFTD1, CFTDM, Fetal isoform of skeletal alpha-actin, G-actin, MPFD, NEM1, NEM2, NEM3, SKA, SKELETAL ALPHA-ACTIN, SKELETAL MUSCLE ACTIN Entrez Gene Name: actin, alpha 1, skeletal muscle Hu Entrez ID: 58, 71 (Related Antibodies: CP64) Mu Entrez ID: 11459 ;-; 11465 Rat Entrez ID: 29437 ;-; 287876

Immunogen

chicken cytoskeletal proteins

Chicken

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Actin is found in all animal cells. Actin monomers have a molecular weight of ~42 kDa. For best results, use the anti-mouse IgM HRP conjugate supplied with the anti-actin antibody. Useful for normalizing protein loading. Antibodies should be titrated for optimal results in individual systems.

Lin, J.J. et al. 1984 in Monoclonal antibodies and functional cell lines. Plenum Publishing Corp.Lin, J.J. 1982. Cold Spring Harbor Symp. on Quant. Biol.46, 769.Lin, J.J. 1981. Proc. Natl. Acad. Sci.78, 2335.Goldman, R.D., et al. 1979. Ann. Rev. Physiol.41, 701.Goldman, R., et al. (eds.) 1976. Cold Spring Harbor Conference on Cell Proliferation, Books A-C.

Packaging

1 ea in Plastic ampoule

Physical form

100 µl ascites diluted in 5 mM sodium phosphate buffer, pH 7.5 and 5 µg anti-mouse IgM, HRP conjugate supplied at 100 µg/ml in 0.05 M sodium phosphate, 50% glycerol.

Reconstitution

Following initial thaw, aliquot ascites and freeze (-20°C).

Warning

Toxicity: Multiple Toxicity Values, refer to MSDS (O)